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ptn5930 cd68  (Bio-Rad)


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    Bio-Rad ptn5930 cd68
    Fig. 2 | Cytokine signaling in the LHb for MORwd plasticity. a, Max-projection of confocal image of the LHb with IBA1+ microglia (left). Max-projection of MedLHb microglia in saline + naloxone and NP-MORwd mice (upper right). 3D reconstruction of IBA1+ microglia containing <t>CD68</t> structures from slices obtained from saline + naloxone and NP-MORwd mice (lower right). b, Analysis of IBA1 microglia immunoreactivity in MedLHb slices (saline + naloxone (n = 4 mice, 366 cells), NP-MORwd (n = 4 mice, 328 cells)). Two-sided t test, t692 = 3.305, ***P = 0.001; 3 independent acquisition sessions. c,d, Quantitative analyses of microglial cell volume (based on IBA1 immunoreactivity) and CD68+ structures. Values are normalized to saline control (saline + naloxone (black; nmice/cells = 4 mice, 89 cells), NP-MORwd (red; n = 4 mice, 83 cells)). IBA1 volume (c): two-sided t test, t170 = 3.05, **P = 0.003. CD68/IBA1 volume (d): two-sided t test, t170 = 2.65, **P = 0.008. Measurements were obtained from independent samples. e, TNF-α (cyan) and DAPI (magenta) immunostaining and normalized TNF-α optical density in the LHb (saline + naloxone (black; n = 7 mice, 6 independent acquisitions per mouse) versus NP-MORwd (red; n = 8 mice, 5 independent acquisitions per mouse)). Two-sided t test, t13 = 2.991, *P = 0.0104. f, Experimental protocol (left) and example traces and AMPAR/NMDAR ratios (right) in the LatLHb with (+) or without (–) exogenous TNF-α (saline + naloxone –TNF-α (n = 2 mice, 9 cells) versus saline + naloxone +TNF-α (n = 2 mice, 9 cells)). Two-sided t test, t14 = 0.37, P = 0.717. g, AMPAR/NMDAR ratios with or without exogenous TNF-α recorded from saline + naloxone (n = 3 mice, 10 cells (–TNF-α) and n = 4 mice, 11 cells (+TNF-α)) and NP-MORwd MedLHb slices (n = 3 mice, 10 cells (–TNF-α) and n = 5 mice, 15 cells (+TNF-α)). Interaction factor F(1,42) = 4.90, two-way ANOVA, *P = 0.039. Data are presented as box plots, with 10th and 90th percentiles, median and scatter.
    Ptn5930 Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptn5930+cd68/pm31209376-296-0-3?v=Bio-Rad
    Average 96 stars, based on 3170 article reviews
    ptn5930 cd68 - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Morphine withdrawal recruits lateral habenula cytokine signaling to reduce synaptic excitation and sociability."

    Article Title: Morphine withdrawal recruits lateral habenula cytokine signaling to reduce synaptic excitation and sociability.

    Journal: Nature neuroscience

    doi: 10.1038/s41593-019-0421-4

    Fig. 2 | Cytokine signaling in the LHb for MORwd plasticity. a, Max-projection of confocal image of the LHb with IBA1+ microglia (left). Max-projection of MedLHb microglia in saline + naloxone and NP-MORwd mice (upper right). 3D reconstruction of IBA1+ microglia containing CD68 structures from slices obtained from saline + naloxone and NP-MORwd mice (lower right). b, Analysis of IBA1 microglia immunoreactivity in MedLHb slices (saline + naloxone (n = 4 mice, 366 cells), NP-MORwd (n = 4 mice, 328 cells)). Two-sided t test, t692 = 3.305, ***P = 0.001; 3 independent acquisition sessions. c,d, Quantitative analyses of microglial cell volume (based on IBA1 immunoreactivity) and CD68+ structures. Values are normalized to saline control (saline + naloxone (black; nmice/cells = 4 mice, 89 cells), NP-MORwd (red; n = 4 mice, 83 cells)). IBA1 volume (c): two-sided t test, t170 = 3.05, **P = 0.003. CD68/IBA1 volume (d): two-sided t test, t170 = 2.65, **P = 0.008. Measurements were obtained from independent samples. e, TNF-α (cyan) and DAPI (magenta) immunostaining and normalized TNF-α optical density in the LHb (saline + naloxone (black; n = 7 mice, 6 independent acquisitions per mouse) versus NP-MORwd (red; n = 8 mice, 5 independent acquisitions per mouse)). Two-sided t test, t13 = 2.991, *P = 0.0104. f, Experimental protocol (left) and example traces and AMPAR/NMDAR ratios (right) in the LatLHb with (+) or without (–) exogenous TNF-α (saline + naloxone –TNF-α (n = 2 mice, 9 cells) versus saline + naloxone +TNF-α (n = 2 mice, 9 cells)). Two-sided t test, t14 = 0.37, P = 0.717. g, AMPAR/NMDAR ratios with or without exogenous TNF-α recorded from saline + naloxone (n = 3 mice, 10 cells (–TNF-α) and n = 4 mice, 11 cells (+TNF-α)) and NP-MORwd MedLHb slices (n = 3 mice, 10 cells (–TNF-α) and n = 5 mice, 15 cells (+TNF-α)). Interaction factor F(1,42) = 4.90, two-way ANOVA, *P = 0.039. Data are presented as box plots, with 10th and 90th percentiles, median and scatter.
    Figure Legend Snippet: Fig. 2 | Cytokine signaling in the LHb for MORwd plasticity. a, Max-projection of confocal image of the LHb with IBA1+ microglia (left). Max-projection of MedLHb microglia in saline + naloxone and NP-MORwd mice (upper right). 3D reconstruction of IBA1+ microglia containing CD68 structures from slices obtained from saline + naloxone and NP-MORwd mice (lower right). b, Analysis of IBA1 microglia immunoreactivity in MedLHb slices (saline + naloxone (n = 4 mice, 366 cells), NP-MORwd (n = 4 mice, 328 cells)). Two-sided t test, t692 = 3.305, ***P = 0.001; 3 independent acquisition sessions. c,d, Quantitative analyses of microglial cell volume (based on IBA1 immunoreactivity) and CD68+ structures. Values are normalized to saline control (saline + naloxone (black; nmice/cells = 4 mice, 89 cells), NP-MORwd (red; n = 4 mice, 83 cells)). IBA1 volume (c): two-sided t test, t170 = 3.05, **P = 0.003. CD68/IBA1 volume (d): two-sided t test, t170 = 2.65, **P = 0.008. Measurements were obtained from independent samples. e, TNF-α (cyan) and DAPI (magenta) immunostaining and normalized TNF-α optical density in the LHb (saline + naloxone (black; n = 7 mice, 6 independent acquisitions per mouse) versus NP-MORwd (red; n = 8 mice, 5 independent acquisitions per mouse)). Two-sided t test, t13 = 2.991, *P = 0.0104. f, Experimental protocol (left) and example traces and AMPAR/NMDAR ratios (right) in the LatLHb with (+) or without (–) exogenous TNF-α (saline + naloxone –TNF-α (n = 2 mice, 9 cells) versus saline + naloxone +TNF-α (n = 2 mice, 9 cells)). Two-sided t test, t14 = 0.37, P = 0.717. g, AMPAR/NMDAR ratios with or without exogenous TNF-α recorded from saline + naloxone (n = 3 mice, 10 cells (–TNF-α) and n = 4 mice, 11 cells (+TNF-α)) and NP-MORwd MedLHb slices (n = 3 mice, 10 cells (–TNF-α) and n = 5 mice, 15 cells (+TNF-α)). Interaction factor F(1,42) = 4.90, two-way ANOVA, *P = 0.039. Data are presented as box plots, with 10th and 90th percentiles, median and scatter.

    Techniques Used: Saline, Control, Immunostaining



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    ptn5930 cd68  (Bio-Rad)
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    Bio-Rad ptn5930 cd68
    Fig. 2 | Cytokine signaling in the LHb for MORwd plasticity. a, Max-projection of confocal image of the LHb with IBA1+ microglia (left). Max-projection of MedLHb microglia in saline + naloxone and NP-MORwd mice (upper right). 3D reconstruction of IBA1+ microglia containing <t>CD68</t> structures from slices obtained from saline + naloxone and NP-MORwd mice (lower right). b, Analysis of IBA1 microglia immunoreactivity in MedLHb slices (saline + naloxone (n = 4 mice, 366 cells), NP-MORwd (n = 4 mice, 328 cells)). Two-sided t test, t692 = 3.305, ***P = 0.001; 3 independent acquisition sessions. c,d, Quantitative analyses of microglial cell volume (based on IBA1 immunoreactivity) and CD68+ structures. Values are normalized to saline control (saline + naloxone (black; nmice/cells = 4 mice, 89 cells), NP-MORwd (red; n = 4 mice, 83 cells)). IBA1 volume (c): two-sided t test, t170 = 3.05, **P = 0.003. CD68/IBA1 volume (d): two-sided t test, t170 = 2.65, **P = 0.008. Measurements were obtained from independent samples. e, TNF-α (cyan) and DAPI (magenta) immunostaining and normalized TNF-α optical density in the LHb (saline + naloxone (black; n = 7 mice, 6 independent acquisitions per mouse) versus NP-MORwd (red; n = 8 mice, 5 independent acquisitions per mouse)). Two-sided t test, t13 = 2.991, *P = 0.0104. f, Experimental protocol (left) and example traces and AMPAR/NMDAR ratios (right) in the LatLHb with (+) or without (–) exogenous TNF-α (saline + naloxone –TNF-α (n = 2 mice, 9 cells) versus saline + naloxone +TNF-α (n = 2 mice, 9 cells)). Two-sided t test, t14 = 0.37, P = 0.717. g, AMPAR/NMDAR ratios with or without exogenous TNF-α recorded from saline + naloxone (n = 3 mice, 10 cells (–TNF-α) and n = 4 mice, 11 cells (+TNF-α)) and NP-MORwd MedLHb slices (n = 3 mice, 10 cells (–TNF-α) and n = 5 mice, 15 cells (+TNF-α)). Interaction factor F(1,42) = 4.90, two-way ANOVA, *P = 0.039. Data are presented as box plots, with 10th and 90th percentiles, median and scatter.
    Ptn5930 Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptn5930+cd68/pm31209376-296-0-3?v=Bio-Rad
    Average 96 stars, based on 1 article reviews
    ptn5930 cd68 - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

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    Fig. 2 | Cytokine signaling in the LHb for MORwd plasticity. a, Max-projection of confocal image of the LHb with IBA1+ microglia (left). Max-projection of MedLHb microglia in saline + naloxone and NP-MORwd mice (upper right). 3D reconstruction of IBA1+ microglia containing CD68 structures from slices obtained from saline + naloxone and NP-MORwd mice (lower right). b, Analysis of IBA1 microglia immunoreactivity in MedLHb slices (saline + naloxone (n = 4 mice, 366 cells), NP-MORwd (n = 4 mice, 328 cells)). Two-sided t test, t692 = 3.305, ***P = 0.001; 3 independent acquisition sessions. c,d, Quantitative analyses of microglial cell volume (based on IBA1 immunoreactivity) and CD68+ structures. Values are normalized to saline control (saline + naloxone (black; nmice/cells = 4 mice, 89 cells), NP-MORwd (red; n = 4 mice, 83 cells)). IBA1 volume (c): two-sided t test, t170 = 3.05, **P = 0.003. CD68/IBA1 volume (d): two-sided t test, t170 = 2.65, **P = 0.008. Measurements were obtained from independent samples. e, TNF-α (cyan) and DAPI (magenta) immunostaining and normalized TNF-α optical density in the LHb (saline + naloxone (black; n = 7 mice, 6 independent acquisitions per mouse) versus NP-MORwd (red; n = 8 mice, 5 independent acquisitions per mouse)). Two-sided t test, t13 = 2.991, *P = 0.0104. f, Experimental protocol (left) and example traces and AMPAR/NMDAR ratios (right) in the LatLHb with (+) or without (–) exogenous TNF-α (saline + naloxone –TNF-α (n = 2 mice, 9 cells) versus saline + naloxone +TNF-α (n = 2 mice, 9 cells)). Two-sided t test, t14 = 0.37, P = 0.717. g, AMPAR/NMDAR ratios with or without exogenous TNF-α recorded from saline + naloxone (n = 3 mice, 10 cells (–TNF-α) and n = 4 mice, 11 cells (+TNF-α)) and NP-MORwd MedLHb slices (n = 3 mice, 10 cells (–TNF-α) and n = 5 mice, 15 cells (+TNF-α)). Interaction factor F(1,42) = 4.90, two-way ANOVA, *P = 0.039. Data are presented as box plots, with 10th and 90th percentiles, median and scatter.

    Journal: Nature neuroscience

    Article Title: Morphine withdrawal recruits lateral habenula cytokine signaling to reduce synaptic excitation and sociability.

    doi: 10.1038/s41593-019-0421-4

    Figure Lengend Snippet: Fig. 2 | Cytokine signaling in the LHb for MORwd plasticity. a, Max-projection of confocal image of the LHb with IBA1+ microglia (left). Max-projection of MedLHb microglia in saline + naloxone and NP-MORwd mice (upper right). 3D reconstruction of IBA1+ microglia containing CD68 structures from slices obtained from saline + naloxone and NP-MORwd mice (lower right). b, Analysis of IBA1 microglia immunoreactivity in MedLHb slices (saline + naloxone (n = 4 mice, 366 cells), NP-MORwd (n = 4 mice, 328 cells)). Two-sided t test, t692 = 3.305, ***P = 0.001; 3 independent acquisition sessions. c,d, Quantitative analyses of microglial cell volume (based on IBA1 immunoreactivity) and CD68+ structures. Values are normalized to saline control (saline + naloxone (black; nmice/cells = 4 mice, 89 cells), NP-MORwd (red; n = 4 mice, 83 cells)). IBA1 volume (c): two-sided t test, t170 = 3.05, **P = 0.003. CD68/IBA1 volume (d): two-sided t test, t170 = 2.65, **P = 0.008. Measurements were obtained from independent samples. e, TNF-α (cyan) and DAPI (magenta) immunostaining and normalized TNF-α optical density in the LHb (saline + naloxone (black; n = 7 mice, 6 independent acquisitions per mouse) versus NP-MORwd (red; n = 8 mice, 5 independent acquisitions per mouse)). Two-sided t test, t13 = 2.991, *P = 0.0104. f, Experimental protocol (left) and example traces and AMPAR/NMDAR ratios (right) in the LatLHb with (+) or without (–) exogenous TNF-α (saline + naloxone –TNF-α (n = 2 mice, 9 cells) versus saline + naloxone +TNF-α (n = 2 mice, 9 cells)). Two-sided t test, t14 = 0.37, P = 0.717. g, AMPAR/NMDAR ratios with or without exogenous TNF-α recorded from saline + naloxone (n = 3 mice, 10 cells (–TNF-α) and n = 4 mice, 11 cells (+TNF-α)) and NP-MORwd MedLHb slices (n = 3 mice, 10 cells (–TNF-α) and n = 5 mice, 15 cells (+TNF-α)). Interaction factor F(1,42) = 4.90, two-way ANOVA, *P = 0.039. Data are presented as box plots, with 10th and 90th percentiles, median and scatter.

    Article Snippet: PTN5930 CD68 1:400, Bio-Rad Cat. MCA1957, Clone FA-11, Lot.

    Techniques: Saline, Control, Immunostaining